David R. Sherwood
Nutritional Regulation of Late Larval Development In collaboration with Dr. Ryan Baugh's lab, we are examining the developmental response of late larvae to starvation. We have identified specific developmental checkpoints that larvae enter in response to the absence of food. These studies have many fascinating implications in our understanding of how cells arrest at specific developmental time-points, how an organism and cells enter and exit quiescent states, and how these impinge on life-span.
Members of our group are trained in a diverse range of scientific approaches and join a vibrant scientific community at Duke University, the Research Triangle region and the worldwide group of worm researchers.
Ph.D., Duke University 1997
B.A., Wesleyan University 1990
Keeley, Daniel P., Eric Hastie, Ranjay Jayadev, Laura C. Kelley, Qiuyi Chi, Sara G. Payne, Jonathan L. Jeger, Brenton D. Hoffman, and David R. Sherwood. “Comprehensive Endogenous Tagging of Basement Membrane Components Reveals Dynamic Movement within the Matrix Scaffolding.” Developmental Cell, June 22, 2020. https://doi.org/10.1016/j.devcel.2020.05.022. Full Text
Hartman, Jessica H., Christopher T. Richie, Kacy L. Gordon, Danielle F. Mello, Priscila Castillo, April Zhu, Yun Wang, et al. “MANF deletion abrogates early larval Caenorhabditis elegans stress response to tunicamycin and Pseudomonas aeruginosa.” European Journal of Cell Biology 98, no. 5–8 (December 2019): 151043. https://doi.org/10.1016/j.ejcb.2019.05.002. Full Text
Jayadev, Ranjay, Qiuyi Chi, Daniel P. Keeley, Eric L. Hastie, Laura C. Kelley, and David R. Sherwood. “α-Integrins dictate distinct modes of type IV collagen recruitment to basement membranes.” The Journal of Cell Biology 218, no. 9 (September 2019): 3098–3116. https://doi.org/10.1083/jcb.201903124. Full Text
Gordon, Kacy L., Sara G. Payne, Lara M. Linden-High, Ariel M. Pani, Bob Goldstein, E Jane Albert Hubbard, and David R. Sherwood. “Ectopic Germ Cells Can Induce Niche-like Enwrapment by Neighboring Body Wall Muscle.” Current Biology : Cb 29, no. 5 (March 2019): 823-833.e5. https://doi.org/10.1016/j.cub.2019.01.056. Full Text
Kelley, Laura C., Qiuyi Chi, Rodrigo Cáceres, Eric Hastie, Adam J. Schindler, Yue Jiang, David Q. Matus, Julie Plastino, and David R. Sherwood. “Adaptive F-Actin Polymerization and Localized ATP Production Drive Basement Membrane Invasion in the Absence of MMPs.” Developmental Cell 48, no. 3 (February 2019): 313-328.e8. https://doi.org/10.1016/j.devcel.2018.12.018. Full Text
Keeley, Daniel P., and David R. Sherwood. “Tissue linkage through adjoining basement membranes: The long and the short term of it.” Matrix Biology : Journal of the International Society for Matrix Biology 75–76 (January 2019): 58–71. https://doi.org/10.1016/j.matbio.2018.05.009. Full Text
Hibshman, Jonathan D., Tess C. Leuthner, Chelsea Shoben, Danielle F. Mello, David R. Sherwood, Joel N. Meyer, and L Ryan Baugh. “Nonselective autophagy reduces mitochondrial content during starvation in Caenorhabditis elegans.” American Journal of Physiology. Cell Physiology 315, no. 6 (December 2018): C781–92. https://doi.org/10.1152/ajpcell.00109.2018. Full Text
Cáceres, Rodrigo, Nagagireesh Bojanala, Laura C. Kelley, Jes Dreier, John Manzi, Fahima Di Federico, Qiuyi Chi, et al. “Forces drive basement membrane invasion in Caenorhabditis elegans.” Proceedings of the National Academy of Sciences of the United States of America 115, no. 45 (November 2018): 11537–42. https://doi.org/10.1073/pnas.1808760115. Full Text
Hartman, Jessica H., Latasha L. Smith, Kacy L. Gordon, Ricardo Laranjeiro, Monica Driscoll, David R. Sherwood, and Joel N. Meyer. “Swimming Exercise and Transient Food Deprivation in Caenorhabditis elegans Promote Mitochondrial Maintenance and Protect Against Chemical-Induced Mitotoxicity.” Scientific Reports 8, no. 1 (May 29, 2018): 8359. https://doi.org/10.1038/s41598-018-26552-9. Full Text Open Access Copy
Sherwood, David R., and Julie Plastino. “Invading, Leading and Navigating Cells in Caenorhabditis elegans: Insights into Cell Movement in Vivo.” Genetics 208, no. 1 (January 2018): 53–78. https://doi.org/10.1534/genetics.117.300082. Full Text
Caceres, R., N. Bojanala, L. C. Kelley, J. Dreier, J. Manzi, F. Di Federico, Q. Chi, et al. “WASP and WAVE activate the Arp2/3 complex for actin-based force production during basement membrane invasion.” In Molecular Biology of the Cell, Vol. 28. AMER SOC CELL BIOLOGY, 2017.
Keeley, D. P., and D. R. Sherwood. “Type IV Collagen stabilizes the connection of juxtaposed tissues at the B-LINK complex.” In Molecular Biology of the Cell, Vol. 27. AMER SOC CELL BIOLOGY, 2016.
Naegeli, K. M., Q. Chi, and D. R. Sherwood. “Rapid exocytosis of an endolysosome-derived membrane domain forms a polarized invasive protrusion that clears basement membrane during cell invasion.” In Molecular Biology of the Cell, Vol. 26. AMER SOC CELL BIOLOGY, 2015.
Jayadev, R., and D. R. Sherwood. “Visualizing and elucidating the regulation of type IV collagen assembly in developing basement membranes.” In Molecular Biology of the Cell, Vol. 26. AMER SOC CELL BIOLOGY, 2015.
Clay, M. R., LR Lilley Lohmer, J. W. Ziel, Q. Chi, and D. R. Sherwood. “Cell invasion through basement membrane requires multiple GTPases and is initiated by CDC-42 directed invadopodia formation.” In Molecular Biology of the Cell, Vol. 25. AMER SOC CELL BIOLOGY, 2014.
Matus, D. Q., L. L. Lohmer, L. C. Kelley, and D. R. Sherwood. “Cell cycle arrest is required for cell invasive behavior.” In Molecular Biology of the Cell, Vol. 25. AMER SOC CELL BIOLOGY, 2014.
Keeley, D. P., M. Morrissey, and D. R. Sherwood. “Investigating the B-LINK: an adhesion system that links neighboring basement membranes.” In Molecular Biology of the Cell, Vol. 25. AMER SOC CELL BIOLOGY, 2014.
Matus, D. Q., E. Chang, S. C. Makohon-Moore, and D. R. Sherwood. “Targeted cell cycle arrest halts basement membrane gap expansion during nematode uterine-vulval attachment.” In Integrative and Comparative Biology, 54:E134–E134. OXFORD UNIV PRESS INC, 2014.
Schindler, A. J., L. R. Baugh, and D. R. Sherwood. “Identification of two novel insulin- and hormone-regulated nutritional checkpoints in C. elegans development.” In Integrative and Comparative Biology, 54:E185–E185. OXFORD UNIV PRESS INC, 2014.
Chang, E., M. Yang, D. R. Sherwood, and D. Q. Matus. “The evolution of anchor cell invasion during rhabditid nematode vulval development.” In Integrative and Comparative Biology, 53:E262–E262. OXFORD UNIV PRESS INC, 2013.
Genetic and Genomics Training Grant awarded by National Institutes of Health (Mentor). 2020 to 2025
Pharmacological Sciences Training Grant awarded by National Institutes of Health (Preceptor). 2020 to 2025
A comprehensive toolkit to visualize endogenously tagged fluorescent basement membrane components in a living animal awarded by National Institutes of Health (Principal Investigator). 2020 to 2022
Understanding the role of the collagen receptor DDR-2 in germ stem cell niche formation awarded by National Institutes of Health (Principal Investigator). 2019 to 2022
Understanding how cells invade through basement membrane in vivo awarded by National Institutes of Health (Principal Investigator). 2016 to 2021
Connecting human disease to basement membrane biology awarded by (Principal Investigator). 2019 to 2020
Transmission electron microscope (TEM) awarded by National Institutes of Health (Minor User). 2019 to 2020
Genetics Training Grant awarded by National Institutes of Health (Mentor). 1979 to 2020
Organization and Function of Cellular Structure awarded by National Institutes of Health (Mentor). 1975 to 2020
Defining a Newly Identified Membrane Structure That Directs Cell Invasion awarded by (Principal Investigator). 2016 to 2019