Nicolas Buchler

Assistant Professor of Biology

Rm 2353a, CIEMAS Bldg, 101 Science Drive, Duke Box 3382, Durham, NC 27710
Campus Box: 
(919) 699-8209
(919) 668-0795

Lab Site:

Our lab is interested in the systems biology and evolution of epigenetic switches (bistability) and clocks (oscillators) in gene regulatory networks, two functions that are essential for patterning, cell proliferation, and differentiation in biological systems. We also study biochemical oscillators such as the cell cycle, metabolic rhythms, and circadian clocks, which co-exist in the same cells and interact with one another through shared resources.


  • Ph.D. 2001, University of Michigan at Ann Arbor

  • M.S. 1998, University of Michigan at Ann Arbor

  • B.S. 1995, University of California at San Diego

Papers Published

Punctuated evolution and transitional hybrid network in an ancestral cell cycle of fungi., 5, 2016
Medina, EM; Turner, JJ; Gordân, R; Skotheim, JM; Buchler, NE, eLife. 5 vol. (MAY2016);

Cell cycle Start is coupled to entry into the yeast metabolic cycle across diverse strains and growth rates., 1, 2016
Burnetti, AJ; Aydin, M; Buchler, NE, Molecular Biology of the Cell. 27 vol. (1); pp. 64-74

Redox rhythm reinforces the circadian clock to gate immune response., 7, 2015
Zhou, M; Wang, W; Karapetyan, S; Mwimba, M; Marqués, J; Buchler, NE; Dong, X, Nature. 523 vol. (7561); pp. 472-476

A noisy linear map underlies oscillations in cell size and gene expression in bacteria., 7, 2015
Tanouchi, Y; Pai, A; Park, H; Huang, S; Stamatov, R; Buchler, NE; You, L, Nature. 523 vol. (7560); pp. 357-360

Role of DNA binding sites and slow unbinding kinetics in titration-based oscillators., 12, 2015
Karapetyan, S; Buchler, NE, Physical Review E - Statistical, Nonlinear, and Soft Matter Physics. 92 vol. (6); pp. 062712

Different Mechanisms Confer Gradual Control and Memory at Nutrient- and Stress-Regulated Genes in Yeast., 11, 2015
Rienzo, A; Poveda-Huertes, D; Aydin, S; Buchler, NE; Pascual-Ahuir, A; Proft, M, Molecular and Cellular Biology. 35 vol. (21); pp. 3669-3683

The Physarum polycephalum Genome Reveals Extensive Use of Prokaryotic Two-Component and Metazoan-Type Tyrosine Kinase Signaling., 11, 2015
Schaap, P; Barrantes, I; Minx, P; Sasaki, N; Anderson, RW; Bénard, M; Biggar, KK; Buchler, NE; Bundschuh, R; Chen, X; Fronick, C; Fulton, L; Golderer, G; Jahn, N; Knoop, V; Landweber, LF; Maric, C; Miller, D; Noegel, AA; Peace, R; Pierron, G; Sasaki, T; Schallenberg-Rüdinger, M; Schleicher, M; Singh, R; Spaller, T; Storey, KB; Suzuki, T; Tomlinson, C; Tyson, JJ; Warren, WC; Werner, ER; Werner-Felmayer, G; Wilson, RK; Winckler, T; Gott, JM; Glöckner, G; Marwan, W, Genome Biology and Evolution. 8 vol. (1); pp. 109-125

Measuring fast gene dynamics in single cells with time-lapse luminescence microscopy., November, 2014
Mazo-Vargas, A; Park, H; Aydin, M; Buchler, NE, Mol Biol Cell. (November, 2014) 25 vol. (22); pp. 3699-3708

Programming stress-induced altruistic death in engineered bacteria., 0, 2012
Tanouchi, Y; Pai, A; Buchler, NE; You, L, Mol Syst Biol. 8 pp. 626

Chromatin: Bind at your own RSC, 0, 2011
Buchler, NE; Bai, L, Curr Biol. 21 vol. (6); pp. R223-R225

Evolution of networks and sequences in eukaryotic cell cycle control, 0, 2011
Cross, FR; Buchler, NE; Skotheim, JM, Philos Trans R Soc Lond B Biol Sci. 366 vol. (1584); pp. 3532-3544

Protein sequestration generates a flexible ultrasensitive response in a genetic network, 0, 2009
Buchler, NE; Cross, FR, Mol Syst Biol. 5 pp. 272

Molecular Titration and Ultrasensitivity in Regulatory Networks, 0, 2008
Buchler, NE; Louis, M, J Mol Biol. 384 vol. (5); pp. 1106-1119

Designing sequential transcription logic: A simple genetic circuit for conditional memory, 0, 2007
Fritz, G; Buchler, NE; Hwa, T; Gerland, U, Systems and Synthetic Biology. 1 vol. (2); pp. 89-98

Nonlinear protein degradation and the function of genetic circuits, 0, 2005
Buchler, NE; Gerland, U; Hwa, T, Proceedings of the National Academy of Sciences of the United States of America. 102 vol. (27); pp. 9559-9564

Two-faced cyclins with eyes on the targets, 0, 2005
Archambault, V; Buchler, NE; Wilmes, GM; Jacobson, MD; Cross, FR, Cell Cycle. 4 vol. (1); pp. 125-130

Transcriptional regulation by the numbers: Applications, 0, 2005
Bintu, L; Buchler, NE; Garcia, HG; Gerland, U; Hwa, T; Kondev, J; Kuhlman, T; Phillips, R, Current Opinion in Genetics and Development. 15 vol. (2); pp. 125-135

Transcriptional regulation by the numbers: Models, 0, 2005
Bintu, L; Buchler, NE; Garcia, HG; Gerland, U; Hwa, T; Kondev, J; Phillips, R, Current Opinion in Genetics and Development. 15 vol. (2); pp. 116-124

On schemes of combinatorial transcription logic, 0, 2003
Buchler, NE; Gerland, U; Hwa, T, Proceedings of the National Academy of Sciences of the United States of America. 100 vol. (9); pp. 5136-5141

Surveying determinants of protein structure designability across different energy models and amino-acid alphabets: A consensus, 2000
Buchler, NEG; Goldstein, RA, Journal of Chemical Physics. (2000) 112 vol. (5); pp. 2533-2547

Effect of alphabet size and foldability requirements on protein structure designability, 0, 1999
Buchler, NEG; Goldstein, RA, Proteins: Structure, Function and Bioinformatics. 34 vol. (1); pp. 113-124

Universal correlation between energy gap and foldability for the random energy model and lattice proteins, October, 1999
Buchler, NEG; Goldstein, RA, Journal of Chemical Physics. (October, 1999) 111 vol. (14); pp. 6599-6609

Protein Heteronuclear NMR Assignments Using Mean-Field Simulated Annealing, 0, 1997
Buchler, NEG; Zuiderweg, ERP; Wang, H; Goldstein, RA, Journal of Magnetic Resonance. 125 vol. (1); pp. 34-42

BL Herculis model pulsations; III. Livermore opacities, 1994
J. R. Buchler and N. E. Buchler, (1994) 285 pp. 213-219


  • 2011 NIH Director's New Innovator Award, National Institutes of Health

  • 2006 Career Award at the Scientific Interface, Burroughs Wellcome Funds