Gustavo M. Silva

Assistant Professor of Biology

Office: 
130 Science Drive, 3103 French, Durham, NC 27708
Campus Box: 
130 Science Drive, Box 90338, Durham, NC 27708
My main research goal is to understand and be able to control how cells respond to stressful and harmful conditions, which are the underlying causes of many human diseases. To achieve this goal, I study cellular response to stress at the protein level and aim to characterize the different regulatory functions mediated by the ubiquitin-proteasome system (UPS), essential machinery involved in modulating protein dynamics. Ultimately, regulating specific UPS roles will provide new tools to increase cellular tolerance to a variety of environmental stresses, which is highly relevant for a variety of degenerative diseases. The main focus of my lab is to investigate the unprecedented regulation of translation mediated by ubiquitin. I laid the groundwork for this research investigating the ubiquitination response in the budding yeast Saccharomyces cerevisiae and we will explore the evolutionary conservation of this pathway and its function in neuronal cells. Our lab is excited to keep pushing the field forward and to use a combination of proteomics, genomics, and molecular methods to understand the mechanisms by which ubiquitin regulates translation, and ultimately, cellular response to stress.

Education

  • Associate Research Scientist, Center For Genomics And Systems Biology, Department Of Biology, New York University 2011 - 2017

  • Ph.D., University of Sao Paulo (Brazil) 2010

  • Lic., University of Sao Paulo (Brazil) 2007

  • B.Sc., University of Sao Paulo (Brazil) 2004

Demasi, Marilene, Luis E. S. Netto, Gustavo M. Silva, Adrian Hand, Cristiano L. P. de Oliveira, Renata N. Bicev, Fabio Gozzo, Mario H. Barros, Janaina M. M. Leme, and Erina Ohara. “Redox regulation of the proteasome via S-glutathionylation.” Redox Biology 2 (2014): 44–51. https://doi.org/10.1016/j.redox.2013.12.003. Full Text

Silva, Gustavo M., Luis E. S. Netto, Vanessa Simões, Luiz F. A. Santos, Fabio C. Gozzo, Marcos A. A. Demasi, Cristiano L. P. Oliveira, et al. “Redox Control of 20S Proteasome Gating.” Antioxidants & Redox Signaling 16, no. 11 (June 2012): 1183–94. https://doi.org/10.1089/ars.2011.4210. Full Text

Vogel, Christine, Gustavo Monteiro Silva, and Edward M. Marcotte. “Protein Expression Regulation under Oxidative Stress.” Molecular & Cellular Proteomics 10, no. 12 (December 2011): M111.009217-M111.009217. https://doi.org/10.1074/mcp.m111.009217. Full Text

Silva, Gustavo M., Luis E. S. Netto, Karen F. Discola, Gilberto M. Piassa-Filho, Daniel C. Pimenta, José A. Bárcena, and Marilene Demasi. “Role of glutaredoxin 2 and cytosolic thioredoxins in cysteinyl-based redox modification of the 20S proteasome.” Febs Journal 275, no. 11 (June 2008): 2942–55. https://doi.org/10.1111/j.1742-4658.2008.06441.x. Full Text

Netto, Luis Eduardo Soares, Marcos Antonio de Oliveira, Gisele Monteiro, Ana Paula Dias Demasi, José Renato Rosa Cussiol, Karen Fulan Discola, Marilene Demasi, et al. “Reactive cysteine in proteins: Protein folding, antioxidant defense, redox signaling and more.” Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology 146, no. 1–2 (July 2007): 180–93. https://doi.org/10.1016/j.cbpc.2006.07.014. Full Text

Discola, Karen Fulan, Marcos Antonio de Oliveira, Gustavo Monteiro Silva, José Antonio Barcena, Pablo Porras, Alicia Padilla, Luis Eduardo Soares Netto, and Beatriz Gomes Guimarães. “Crystallization and preliminary X-ray crystallographic studies of glutaredoxin 2 from Saccharomyces cerevisiae in different oxidation states.Acta Crystallographica. Section F, Structural Biology and Crystallization Communications 61, no. Pt 4 (April 2005): 445–47. https://doi.org/10.1107/s1744309105008730. Full Text

Demasi, Marilene, Gustavo Monteiro Silva, and Luis Eduardo Soares Netto. “20 S Proteasome fromSaccharomyces cerevisiaeIs Responsive to Redox Modifications and IsS-Glutathionylated.” Journal of Biological Chemistry 278, no. 1 (January 3, 2003): 679–85. https://doi.org/10.1074/jbc.m209282200. Full Text

Tchourine, Konstantine, Christopher S. Poultney, Li Wang, Gustavo M. Silva, Sandhya Manohar, Christian L. Mueller, Richard Bonneau, and Christine Vogel. “One third of dynamic protein expression profiles can be predicted by a simple rate equation.” Mol. Biosyst. 10, no. 11 (n.d.): 2850–62. https://doi.org/10.1039/c4mb00358f. Full Text

Malvezzi, Alberto, Patrícia M. Higa, Antonia T-do Amaral, Gustavo M. Silva, Fabio C. Gozzo, Emer S. Ferro, Leandro M. Castro, Leandro de Rezende, Gisele Monteiro, and Marilene Demasi. “The Cysteine-Rich Protein Thimet Oligopeptidase as a Model of the Structural Requirements for S-glutathiolation and Oxidative Oligomerization.” Plos One 7, no. 6 (n.d.): e39408–e39408. https://doi.org/10.1371/journal.pone.0039408. Full Text

Pages

Fellowships, Supported Research, & Other Grants