Concepts and theory involved in a wide variety of microscopy and image analysis. The course is intended for people who will do a significant amount of biological imaging in their graduate research and is best taken after starting to do some imaging. Areas covered: Transmitted light, fluorescence, widefield imaging, scanning confocal, TIRF, live-cell imaging, multiphoton excitation, spinning disk, super-resolution microscopy, imaging techniques, image processing, visualization and analysis. FIJI/ImageJ is used with interactive exercises to complement theory involved in image processing and analysis. Consent of the instructor is required (numbers are limited).