Nina Tang Sherwood

Associate Professor of the Practice in the Department of Biology

Office: 
Box 90338 137 Bio Sciences, 130 Science Drive, Durham, NC 27708
Campus Box: 
Duke Box 90338, Science Drive, 371D Biosci, Durham, NC 27708
Phone: 
(919) 684-8658
We use Drosophila melanogaster as a model to understand nervous system development and function. A genetic screen for molecules important to these processes identified the fly ortholog of the spastin gene, which when mutated in humans leads to a progressive neurodegenerative disease called Autosomal-Dominant Hereditary Spastic Paraplegia (AD-HSP). Individuals with AD-HSP have difficulty walking, sometimes from as early as childhood, and can end up confined to wheelchairs. We have shown that loss of spastin in the fly larva compromises motoneuron function, while adults exhibit weak legs and do not fly. Spastin is a member of the AAA family of ATPases, and functions by severing microtubules into smaller segments. Our results indicate that the absence of spastin function in mutant flies leads to a reduction in microtubule content at synaptic boutons, presumably causing the weakened neurotransmission. Among our goals in the lab are to understand how this happens at a cell-biological level, and to examine specific phenotypes associated with mutations mimicking those found in the human disease. Using Drosophila as a model system allows us to rapidly generate flies with any number of specific mutations, and then study the consequences of these mutations at the biochemical, cell biological, developmental, electrophysiological and behavioral levels.

Education

  • Ph.D., Duke University 1998

  • B.S., University of California at San Diego 1990

Ozdowski, EF, Baxter, SL, and Sherwood, NT. "Drosophila Models of Hereditary Spastic Paraplegia." In Movement Disorders: Genetics and Models: Second Edition,1103-1122. October 29, 2014. Full Text

Baxter, SL, Allard, DE, Crowl, C, and Sherwood, NT. "Cold temperature improves mobility and survival in Drosophila models of autosomal-dominant hereditary spastic paraplegia (AD-HSP)." Disease models & mechanisms 7, no. 8 (August 2014): 1005-1012. Full Text

Stewart, A, Tsubouchi, A, Rolls, MM, Tracey, WD, and Sherwood, NT. "Katanin p60-like1 promotes microtubule growth and terminal dendrite stability in the larval class IV sensory neurons of Drosophila." J Neurosci 32, no. 34 (August 22, 2012): 11631-11642. Full Text

Stone, MC, Rao, K, Gheres, KW, Kim, S, Tao, J, Rochelle, CL, Folker, CT, Sherwood, NT, and Rolls, MM. "Normal Spastin Gene Dosage Is Specifically Required for Axon Regeneration." Cell Reports 2, no. 5 (2012): 1340-1350. Full Text

Ozdowski, EF, Gayle, S, Bao, H, Zhang, B, and Sherwood, NT. "Loss of Drosophila melanogaster p21-activated kinase 3 suppresses defects in synapse structure and function caused by spastin mutations." Genetics 189, no. 1 (September 2011): 123-135. Full Text

Du, F, Ozdowski, EF, Kotowski, IK, Marchuk, DA, and Sherwood, NT. "Functional conservation of human Spastin in a Drosophila model of autosomal dominant-hereditary spastic paraplegia." Hum Mol Genet 19, no. 10 (May 15, 2010): 1883-1896. Full Text

Sherwood, NT, Sun, Q, Xue, M, Zhang, B, and Zinn, K. "Drosophila spastin regulates synaptic microtubule networks and is required for normal motor function." PLoS Biology 2, no. 12 (2004). Full Text

Sherwood, NT, and Lo, DC. "Long-term enhancement of central synaptic transmission by chronic brain-derived neurotrophic factor treatment." J Neurosci 19, no. 16 (August 15, 1999): 7025-7036.

Sherwood, NT, Lesser, SS, and Lo, DC. "Neurotrophin regulation of ionic currents and cell size depends on cell context." Proc Natl Acad Sci U S A 94, no. 11 (May 27, 1997): 5917-5922.

Lesser, SS, Sherwood, NT, and Lo, DC. "Neurotrophins differentially regulate voltage-gated ion channels." Mol Cell Neurosci 10, no. 3-4 (1997): 173-183. Full Text

Genetic Analysis of the Roles and Regulation of Microtubule Severing in Neurons awarded by National Institutes of Health (Principal Investigator). 2008 to 2012

Neurotrophin Modulation Of Synaptic Transmission awarded by National Institutes of Health (Principal Investigator). 1995 to 1997

Neurotrophin Modulation Of Synaptic Transmission awarded by National Institutes of Health (Principal Investigator). 1996 to 1997