James N. Siedow

Professor Emeritus of Biology

Office: 
130 Science Drive, 3105 French Science Centre, Durham, NC 27708
Campus Box: 
Duke Box 90338, Department of Biology, Durham, NC 27708
Phone: 
(919) 684-5445
Physiological, biochemical and molecular studies of plant oxidative processes. I suspended the operation of my laboratory about eight years ago. Historically, the research in my laboratory looked at metabolic processes related to aerobic respiration in plants and fungi. Specifically, this research involves isolating and characterizing the structural and regulatory features of the cyanide-resistant "alternative" oxidase associated with all plant and many fungal mitochondria. The mechanism of action of a regulatory sulfhydryl-disulfide system on the alternative oxidase and its interplay with the activating effects of alpha-keto acids are currently under study, as is the characterization of structural features of the alternative protein and its active site. Comparative studies of the plant and fungal alternative oxidases have indicated a common structural framework but distinctly different regulatory features. This research involves the application of standard biochemical approaches, including chemical cross-linking, immunochemical technologies, and electrochemical methodologies. These approaches are further complemented by the use of site-directed mutagenesis of the alternative oxidase expressed in both the bacterium E. coli and transgenic Arabidopsis thaliana. Transformed Arabidopsis having over- and underexpressed wild-type, as well as altered forms of the alternative oxidase lacking normal regulatory control have been developed and are being used to characterize the role played by the alternative oxidase in response to different developmental and environmental circumstances, including extremes of temperature, pathogen infection, and nutrient limitations.

Education

  • Ph.D., Indiana University at Indianapolis 1972

  • B.A., University of Texas at Austin 1969

Yuan, F, Yang, H, Xue, Y, Kong, D, Ye, R, Li, C, Zhang, J, Theprungsirikul, L, Shrift, T, Krichilsky, B, Johnson, DM, Swift, GB, He, Y, Siedow, JN, and Pei, ZM. "Corrigendum: OSCA1 mediates osmotic-stress-evoked Ca(2+) increases vital for osmosensing in Arabidopsis." Nature (February 2015).

Yuan, F, Yang, H, Xue, Y, Kong, D, Ye, R, Li, C, Zhang, J, Theprungsirikul, L, Shrift, T, Krichilsky, B, Johnson, DM, Swift, GB, He, Y, Siedow, JN, and Pei, Z-M. "OSCA1 mediates osmotic-stress-evoked Ca2+ increases vital for osmosensing in Arabidopsis." Nature 514, no. 7522 (October 2014): 367-371. Full Text

Wu, X, Wu, F-H, Wang, X, Wang, L, Siedow, JN, Zhang, W, and Pei, Z-M. "Molecular evolutionary and structural analysis of the cytosolic DNA sensor cGAS and STING." Nucleic acids research 42, no. 13 (July 2014): 8243-8257. Full Text

Florez-Sarasa, I, Flexas, J, Rasmusson, AG, Umbach, AL, Siedow, JN, and Ribas-Carbo, M. "In vivo cytochrome and alternative pathway respiration in leaves of Arabidopsis thaliana plants with altered alternative oxidase under different light conditions." Plant Cell Environ 34, no. 8 (August 2011): 1373-1383. Full Text

Moller, IM, Rasmusson, AG, Siedow, JJN, and Vanlerberghe, GC. "The product of the alternative oxidase is still H2O." ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 495, no. 1 (March 1, 2010): 93-94. Full Text

Møller, IM, Rasmusson, AG, Siedow, JN, and Vanlerberghe, GC. "The product of the alternative oxidase is still H2O." Archives of Biochemistry and Biophysics 495, no. 1 (2010): 93-94. Full Text

Rhoads, DM, Umbach, AL, Subbaiah, CC, and Siedow, JN. "Mitochondrial reactive oxygen species. Contribution to oxidative stress and interorganellar signaling." Plant Physiol 141, no. 2 (June 2006): 357-366. Full Text

Umbach, AL, Ng, VS, and Siedow, JN. "Regulation of plant alternative oxidase activity: a tale of two cysteines." Biochim Biophys Acta 1757, no. 2 (February 2006): 135-142. Full Text

Pages

High-performance Computing System for Bioinformatics awarded by National Institutes of Health (Advisor). 2009 to 2010

Studies of the Cyanide-Resistant Alternative Oxidase awarded by National Science Foundation (Principal Investigator). 1997 to 2005

Iain Hamilton Sculpture Events awarded by The Mary Duke Biddle Foundation (Principal Investigator). 2002 to 2003

Oxygen Isotope Fractionation by the Cyanide-Resistant Oxidase in Plants awarded by Cooperative State Research Service (CSRS) (Principal Investigator). 1999 to 2002

BioTerrorism Workshop awarded by Army Research Office (Principal Investigator). 2002

(94-0718) Acquisition of Growth Chambers for the National Phytotron awarded by National Science Foundation (Co-Principal Investigator). 1994 to 1999

(96-0340) Oxygen Isotype Fractionation in Plant Mitochondria awarded by Cooperative State Research Service (CSRS) (Principal Investigator). 1994 to 1999

(94-0817) Continuation of Operations of the National Phytotron awarded by National Science Foundation (Co-Principal Investigator). 1995 to 1999

(97-0507) Studies of the Plant Cyanide - Resistant Alternative Oxidase awarded by National Science Foundation (Principal Investigator). 1997 to 1998

(96-0878) Studies of the Cyanide-resistant Respiratory Pathways awarded by National Science Foundation (Principal Investigator). 1994 to 1997

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