Daniel P. Kiehart

Professor of Biology

Office: 
4330 French Family Science Center, Science Drive, Duke University, Durham, NC 27
Campus Box: 
90338
Phone: 
(919) 613-8157
Fax: 
919-660-7293 (not secure fax)

Lab Site: http://www.biology.duke.edu/kiehartlab/

Research Interests: 

Our intellectual focus is on identifying determinants of cell shape that function during development and wound healing. We utilize novel biophysical strategies (in collaboration with Glenn Edwards' group in Physics and with Stephanos Venakide's and John Harer's groups in Mathematics) in concert with modern molecular genetic and reverse genetic approaches in Drosophila to explore the forces that are responsible for cell shape change and movements. We show that both the amnioserosa and a "supracellular purse string" in the leading edge of the lateral epidermis contribute to the movements of dorsal closure. Dorsal closure proceeds even if we ablate one (but NOT both!)of the tissues responsible for closure, indicating that this model cell (epithelial) sheet movement depends on redundant forces that in concert drive morphogenesis. We show that the magnitude of each force is significantly larger than their vector sum indicating that there is both potential for generating large forces and that successful morphogenesis requires that the forces applied be precisely balanced. We have also explored the molecules responsible for generating those movements. We showed that conventional nonmuscle myosin (myosin II) provides key contractile forces in different tissues where the supramolecular complexes that incorporate this motor protein are distinct. How molecular events are regulated such that large, opposing forces efficiently drive morphogenesis remains a mystery, but we are pursuing leads that point to two distinct pathways: the bidirectionally signaling integrin cell surface receptors and mechanically gated channels. We are also pursuing the morphogenesis of actin-cytoskeleton based projections that are a key feature of a variety of cells, including those that are specialized for sensory reception in human vision and hearing. We have again turned to Drosophila as a model system where we study the morphogenesis of epidermal hairs and sensory bristles. Our work centers on an unconventional myosin (myosin VIIA) encoded by crinkled a gene that is required for the formation of epidermal hairs and bristles. We show that myosin VIIA is required for the coallescence of actin pre-hairs into the robust actin bundles that form the skeleton on which hairs and bristles can be built. In collaboration with Dan Eberl's lab (University of Iowa) we showed that myosin VIIA is also essential for fly hearing -- remarkably, its human homolog is also required for human hearing, even though the mechanisms of auditory sensory reception in these phylogenetically diverged systems are very different. We have begun to characterize myosin VIIA structurally using NMR of purified protein domains. With Jim Seller's lab at the NIH we have used fast time course kinetics and single molecule assays to analyze molecular function and show that this myosin VIIA is a processive motor. We are beginning to characterize the proteins that collaborate with both myosin II and myosin VIIA using biochemical strategies in vitro, yeast two hybrid approaches in vivo and genetic interaction strategies in fly. Together, our experiments promise to reveal the nature of cytoskeletal function in cell shape determination for cell division and morphogenesis throughout development and organismal homeostasis.

Our intellectual focus is on identifying determinants of cell shape that function during development. Utilizing molecular genetic and reverse genetic approaches in Drosophila, we have shown that conventional nonmuscle myosin is necessary for driving both cell division and post-mitotic cell shape changes for morphogenesis, and cellular locomotions. Currently, we are investigating how myosin elicits cell shape change and how its function is regulated through filament formation, phosphorylation, sub-cellular targeting and small GTP-binding protein function. We are characterizing myosin light chain kinase; a novel myosin VII heavy chain; and additional elements that participate in localizing myosin and transmitting the forces that it produces. We used screens for aberrant cell shape induced in the yeast S. pombe by expression of transfected Drosophila cDNAs. These experiments show that elements that define cell shape are conserved throughout phylogeny and that a screen in yeast is a valuable tool for recovering heterologous cDNAs that encode cytoskeletal elements and the proteins that regulate them. In fly, we are identifying gene products that are necessary for myosin function by genetically recovering second site non-complementing loci and biochemically recovering proteins that bind to myosin. To date, our experiments identify ~30 loci that genetically interact with myosin and a kinase activity that phosphorylates myosin heavy chain and establish genetically, that the Rho signalling pathway is required in concert with nonmuscle myosin II for morphogenesis. We are also using manipulation studies to understand the forces that drive cellularization and morphogenesis. We show that both the amnioserosa and the leading edge of the lateral epidermis contribute to the movements of dorsal closure. Finally, we are examining the role these proteins play in movements that occur during wound healing.

Education

  • Postdoctoral Fellow 1982, Johns Hopkins University Medical School (Thomas D. Pollard, Advisor)

  • Ph.D. 1979, University of Pennsylvania

  • B.A. 1973, University of Pennsylvania

Papers Published

MRCK-1 Drives Apical Constriction in C. elegans by Linking Developmental Patterning to Force Generation., 8, 2016
Marston, DJ; Higgins, CD; Peters, KA; Cupp, TD; Dickinson, DJ; Pani, AM; Moore, RP; Cox, AH; Kiehart, DP; Goldstein, B, Current Biology. 26 vol. (16); pp. 2079-2089

Quantifying dorsal closure in three dimensions., 12, 2016
Lu, H; Sokolow, A; Kiehart, DP; Edwards, GS, Molecular Biology of the Cell. 27 vol. (25); pp. 3948-3955

Moving Inward: Establishing the Mammalian Inner Cell Mass., 8, 2015
Goldstein, B; Kiehart, DP, Developmental Cell. 34 vol. (4); pp. 385-386

Epithelial morphogenesis: apoptotic forces drive cell shape changes., 3, 2015
Kiehart, DP, Developmental Cell. 32 vol. (5); pp. 532-533

Remodeling Tissue Interfaces and the Thermodynamics of Zipping during Dorsal Closure in Drosophila., 12, 2015
Lu, H; Sokolow, A; Kiehart, DP; Edwards, GS, Biophysical Journal. 109 vol. (11); pp. 2406-2417

Complete canthi removal reveals that forces from the amnioserosa alone are sufficient to drive dorsal closure in Drosophila., 11, 2014
Wells, AR; Zou, RS; Tulu, US; Sokolow, AC; Crawford, JM; Edwards, GS; Kiehart, DP, Molecular Biology of the Cell. 25 vol. (22); pp. 3552-3568

Lattice light-sheet microscopy: imaging molecules to embryos at high spatiotemporal resolution., 10, 2014
Chen, B-C; Legant, WR; Wang, K; Shao, L; Milkie, DE; Davidson, MW; Janetopoulos, C; Wu, XS; Hammer, JA; Liu, Z; English, BP; Mimori-Kiyosue, Y; Romero, DP; Ritter, AT; Lippincott-Schwartz, J; Fritz-Laylin, L; Mullins, RD; Mitchell, DM; Bembenek, JN; Reymann, A-C; Böhme, R; Grill, SW; Wang, JT; Seydoux, G; Tulu, US; Kiehart, DP; Betzig, E, Science. 346 vol. (6208); pp. 1257998

Ion channels contribute to the regulation of cell sheet forces during Drosophila dorsal closure., 1, 2014
Hunter, GL; Crawford, JM; Genkins, JZ; Kiehart, DP, Development (Cambridge). 141 vol. (2); pp. 325-334

Cell ingression and apical shape oscillations during dorsal closure in Drosophila., January 17, 2012
Sokolow, A; Toyama, Y; Kiehart, DP; Edwards, GS, Biophysical Journal. (January 17, 2012) 102 vol. (5); pp. 969-979

Triggering a cell shape change by exploiting preexisting actomyosin contractions., January 20, 2012
Roh-Johnson, M; Shemer, G; Higgins, CD; McClellan, JH; Werts, AD; Tulu, US; Gao, L; Betzig, E; Kiehart, DP; Goldstein, B, Science. (January 20, 2012) 335 vol. (6073); pp. 1232-1235

Nonmuscle myosin II is required for cell proliferation, cell sheet adhesion and wing hair morphology during wing morphogenesis., 9, 2010
Franke, JD; Montague, RA; Kiehart, DP, Dev Biol. 345 vol. (2); pp. 117-132

Division of labor: subsets of dorsal-appendage-forming cells control the shape of the entire tube., 10, 2010
Boyle, MJ; French, RL; Cosand, KA; Dorman, JB; Kiehart, DP; Berg, CA, Dev Biol. 346 vol. (1); pp. 68-79

Drosophila morphogenesis: tissue force laws and the modeling of dorsal closure., 12, 2009
Layton, AT; Toyama, Y; Yang, G-Q; Edwards, GS; Kiehart, DP; Venakides, S, HFSP J. 3 vol. (6); pp. 441-460

Apoptotic force and tissue dynamics during Drosophila embryogenesis., 9, 2008
Toyama, Y; Peralta, XG; Wells, AR; Kiehart, DP; Edwards, GS, Science. 321 vol. (5896); pp. 1683-1686

Actomyosin purse strings: renewable resources that make morphogenesis robust and resilient., 8, 2008
Rodriguez-Diaz, A; Toyama, Y; Abravanel, DL; Wiemann, JM; Wells, AR; Tulu, US; Edwards, GS; Kiehart, DP, HFSP Journal. 2 vol. (4); pp. 220-237

Myosin VIIA, important for human auditory function, is necessary for Drosophila auditory organ development., 5, 2008
Todi, SV; Sivan-Loukianova, E; Jacobs, JS; Kiehart, DP; Eberl, DF, PloS one. 3 vol. (5); pp. e2115

Emergent properties during dorsal closure in Drosophila morphogenesis., 4, 2008
Peralta, XG; Toyama, Y; Kiehart, DP; Edwards, GS, Physical Biology. 5 vol. (1); pp. 015004

Emergent properties during dorsal closure in Drosophila morphogenesis., 0, 2008
Peralta, XG; Toyama, Y; Kiehart, DP; Edwards, GS, Physical Biology. 5 vol. (1); pp. 15004

Upregulation of forces and morphogenic asymmetries in dorsal closure during Drosophila development., 4, 2007
Peralta, XG; Toyama, Y; Hutson, MS; Montague, R; Venakides, S; Kiehart, DP; Edwards, GS, Biophysical Journal. 92 vol. (7); pp. 2583-2596

An MYH9 human disease model in flies: site-directed mutagenesis of the Drosophila non-muscle myosin II results in hypomorphic alleles with dominant character., 12, 2007
Franke, JD; Montague, RA; Rickoll, WL; Kiehart, DP, Human Molecular Genetics. 16 vol. (24); pp. 3160-3173

Cell structure and dynamics, February, 2007
Kiehart, DP; Bloom, K, Current Opinion in Cell Biology. (February, 2007) 19 vol. (1); pp. 1-4

Dimerized Drosophila myosin VIIa: a processive motor., 4, 2006
Yang, Y; Kovács, M; Sakamoto, T; Zhang, F; Kiehart, DP; Sellers, JR, Proceedings of the National Academy of Sciences of USA. 103 vol. (15); pp. 5746-5751

JNK signaling coordinates integrin and actin functions during Drosophila embryogenesis., 2, 2006
Homsy, JG; Jasper, H; Peralta, XG; Wu, H; Kiehart, DP; Bohmann, D, Developmental Dynamics. 235 vol. (2); pp. 427-434

Native nonmuscle myosin II stability and light chain binding in Drosophila melanogaster., 10, 2006
Franke, JD; Boury, AL; Gerald, NJ; Kiehart, DP, Cell Motility and the Cytoskeleton. 63 vol. (10); pp. 604-622

Myosin VIIA defects, which underlie the Usher 1B syndrome in humans, lead to deafness in Drosophila., 5, 2005
Todi, SV; Franke, JD; Kiehart, DP; Eberl, DF, Current Biology. 15 vol. (9); pp. 862-868

Nonmuscle myosin II generates forces that transmit tension and drive contraction in multiple tissues during dorsal closure., 12, 2005
Franke, JD; Montague, RA; Kiehart, DP, Current Biology. 15 vol. (24); pp. 2208-2221

Rod mutations associated with MYH9-related disorders disrupt nonmuscle myosin-IIA assembly., January, 2005
Franke, JD; Dong, F; Rickoll, WL; Kelley, MJ; Kiehart, DP, Blood. (January, 2005) 105 vol. (1); pp. 161-169

bullwinkle is required for epithelial morphogenesis during Drosophila oogenesis., March 15, 2004
Dorman, JB; James, KE; Fraser, SE; Kiehart, DP; Berg, CA, Developmental Biology. (March 15, 2004) 267 vol. (2); pp. 320-341

Drosophila crinkled, mutations of which disrupt morphogenesis and cause lethality, encodes fly myosin VIIA., 2004
Kiehart, DP; Franke, JD; Chee, MK; Montague, RA; Chen, T-L; Roote, J; Ashburner, M, Genetics. (2004) 168 vol. (3); pp. 1337-1352

Forces for morphogenesis investigated with laser microsurgery and quantitative modeling., 4, 2003
Hutson, MS; Tokutake, Y; Chang, M-S; Bloor, JW; Venakides, S; Kiehart, DP; Edwards, GS, Science. 300 vol. (5616); pp. 145-149

Myosins motor Miranda., 12, 2003
Kiehart, DP, Molecular Cell. 12 vol. (6); pp. 1346-1347

Genetic interactions between the RhoA and Stubble-stubbloid loci suggest a role for a type II transmembrane serine protease in intracellular signaling during Drosophila imaginal disc morphogenesis., 11, 2003
Bayer, CA; Halsell, SR; Fristrom, JW; Kiehart, DP; von Kalm, L, Genetics. 165 vol. (3); pp. 1417-1432

Free-electron-laser-based biophysical and biomedical instrumentation, 0, 2003
Edwards, GS; Austin, RH; Carroll, FE; Copeland, ML; Couprie, ME; Gabella, WE; Huglund, RF; Hooper, BA; Hutson, MS; Jansen, ED; Joos, KM; Kiehart, DP; Lindau, I; Miao, J; Pratisto, HS; Shen, JH; Tokutake, Y; Meer, AFGVD; Xie, A, Review of Scientific Instruments. 74 vol. (7); pp. 3207-3245

FEL-based biophysical and biomedical instrumentation, 0, 2003
Edwards, GS; Austin, RH; Carroll, FE; Copeland, ML; Couprie, ME; Gabella, WE; Haglund, RF; Hooper, BA; Hutson, MS; Jansen, ED; Joos, KM; Kiehart, DP; Lindau, I; Miao, J; Pratisto, HS; Shen, JH; Tokutake, Y; van Der Meer, L; Xie, A, Invited paper, Review of Scientific Instruments. 74 vol. (7); pp. 3207-3245

Real-time imaging of morphogenetic movements in Drosophila using Gal4-UAS-driven expression of GFP fused to the actin-binding domain of moesin., 9, 2002
Dutta, D; Bloor, JW; Ruiz-Gomez, M; VijayRaghavan, K; Kiehart, DP, Genesis. 34 vol. (1-2); pp. 146-151

Actin dynamics: the arp2/3 complex branches out., 8, 2002
Kiehart, DP; Franke, JD, Current Biology. 12 vol. (16); pp. R557-R559

Drosophila RhoA regulates the cytoskeleton and cell-cell adhesion in the developing epidermis., 7, 2002
Bloor, JW; Kiehart, DP, Development (Cambridge). 129 vol. (13); pp. 3173-3183

Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants, 0, 2002
Ohashi, T; Kiehart, DP; Erickson, HP, Journal of Cell Science. 115 vol. (6); pp. 1221-1229

Role of myosin-II phosphorylation in V12Cdc42-mediated disruption of Drosophila cellularization., 3, 2001
Crawford, JM; Su, Z; Varlamova, O; Bresnick, AR; Kiehart, DP, European Journal of Cell Biology. 80 vol. (3); pp. 240-244

Protein kinase C phosphorylates nonmuscle myosin-II heavy chain from Drosophila but regulation of myosin function by this enzyme is not required for viability in flies., 3, 2001
Su, Z; Kiehart, DP, Biochemistry. 40 vol. (12); pp. 3606-3614

zipper Nonmuscle myosin-II functions downstream of PS2 integrin in Drosophila myogenesis and is necessary for myofibril formation., 11, 2001
Bloor, JW; Kiehart, DP, Developmental Biology. 239 vol. (2); pp. 215-228

With Malice Towards None, 0, 2001
DP Kiehart, Raleigh News and Observer.

Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during Drosophila morphogenesis., 7, 2000
Halsell, SR; Chu, BI; Kiehart, DP, Genetics. 155 vol. (3); pp. 1253-1265

Drosophila stretchin-MLCK is a novel member of the Titin/Myosin light chain kinase family., 7, 2000
Champagne, MB; Edwards, KA; Erickson, HP; Kiehart, DP, Journal of Molecular Biology. 300 vol. (4); pp. 759-777

Multiple forces contribute to cell sheet morphogenesis for dorsal closure in Drosophila., 4, 2000
Kiehart, DP; Galbraith, CG; Edwards, KA; Rickoll, WL; Montague, RA, The Journal of Cell Biology. 149 vol. (2); pp. 471-490

Erratum: Genetic analysis demonstrates a direct link between rho signaling and nonmuscle myosin function during drosophila morphogenesis (Genetics 155 (1253-1265)), 0, 2000
Halsell, SR; Chu, BI; Kiehart, DP, Genetics. 156 vol. (1); pp. 469-

Wound healing: The power of the purse string., 8, 1999
Kiehart, DP, Current Biology. 9 vol. (16); pp. R602-R605

Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-green fluorescent protein., 3, 1999
Ohashi, T; Kiehart, DP; Erickson, HP, Proceedings of the National Academy of Sciences of USA. 96 vol. (5); pp. 2153-2158

From one cell to many, 0, 1999
Crawford, JM; Kiehart, DP, Current Biology. 9 vol. (11); pp. R389

Drosophila betaHeavy-spectrin is essential for development and contributes to specific cell fates in the eye., 6, 1998
Thomas, GH; Zarnescu, DC; Juedes, AE; Bales, MA; Londergan, A; Korte, CC; Kiehart, DP, Development (Cambridge). 125 vol. (11); pp. 2125-2134

Second-site noncomplementation identifies genomic regions required for Drosophila nonmuscle myosin function during morphogenesis., 4, 1998
Halsell, SR; Kiehart, DP, Genetics. 148 vol. (4); pp. 1845-1863

Cellularization in Drosophila melanogaster is disrupted by the inhibition of rho activity and the activation of Cdc42 function., 12, 1998
Crawford, JM; Harden, N; Leung, T; Lim, L; Kiehart, DP, Developmental Biology. 204 vol. (1); pp. 151-164

Volume changes induced by osmotic stress in freshly isolated rat hippocampal neurons., 11, 1998
Aitken, PG; Borgdorff, AJ; Juta, AJ; Kiehart, DP; Somjen, GG; Wadman, WJ, Pflügers Archiv. 436 vol. (6); pp. 991-998

Intragenic duplication and divergence in the spectrin superfamily of proteins., 12, 1997
Thomas, GH; Newbern, EC; Korte, CC; Bales, MA; Muse, SV; Clark, AG; Kiehart, DP, Molecular Biology and Evolution. 14 vol. (12); pp. 1285-1295

GFP-moesin illuminates actin cytoskeleton dynamics in living tissue and demonstrates cell shape changes during morphogenesis in Drosophila., 11, 1997
Edwards, KA; Demsky, M; Montague, RA; Weymouth, N; Kiehart, DP, Developmental Biology. 191 vol. (1); pp. 103-117

Septins may form a ubiquitous family of cytoskeletal filaments., 9, 1996
Cooper, JA; Kiehart, DP, The Journal of Cell Biology. 134 vol. (6); pp. 1345-1348

Drosophila nonmuscle myosin II has multiple essential roles in imaginal disc and egg chamber morphogenesis., 5, 1996
Edwards, KA; Kiehart, DP, Development (Cambridge). 122 vol. (5); pp. 1499-1511

The role of HOM-C genes in segmental transformations: reexamination of the Drosophila Sex combs reduced embryonic phenotype., 11, 1996
Pederson, JD; Kiehart, DP; Mahaffey, JW, Developmental Biology. 180 vol. (1); pp. 131-142

Molecular organization and alternative splicing in zipper, the gene that encodes the Drosophila non-muscle myosin II heavy chain., 1, 1996
Mansfield, SG; al-Shirawi, DY; Ketchum, AS; Newbern, EC; Kiehart, DP, Journal of Molecular Biology. 255 vol. (1); pp. 98-109

Fly division., 10, 1995
Miller, KG; Kiehart, DP, The Journal of Cell Biology. 131 vol. (1); pp. 1-5

Essential light chain of Drosophila nonmuscle myosin II., 10, 1995
Edwards, KA; Chang, XJ; Kiehart, DP, Journal of Muscle Research and Cell Motility. 16 vol. (5); pp. 491-498

Beta heavy-spectrin has a restricted tissue and subcellular distribution during Drosophila embryogenesis., 7, 1994
Thomas, GH; Kiehart, DP, Development (Cambridge). 120 vol. (7); pp. 2039-2050

Identification of Drosophila cytoskeletal proteins by induction of abnormal cell shape in fission yeast., 5, 1994
Edwards, KA; Montague, RA; Shepard, S; Edgar, BA; Erikson, RL; Kiehart, DP, Proceedings of the National Academy of Sciences of USA. 91 vol. (10); pp. 4589-4593

β<sub>Havy</sub>-spectrin has a restricted tissue and subcellular distribution during Drosophila embryogenesis, 0, 1994
Thomas, GH; Kiehart, DP, Development. 120 vol. (7); pp. 2039-2050

High-resolution microscopic methods for the analysis of cellular movements in Drosophila embryos., 0, 1994
Kiehart, DP; Montague, RA; Rickoll, WL; Foard, D; Thomas, GH, Methods in cell biology. 44 pp. 507-532

High-resolution microscopic methods for the analysis of cellular movements in Drosophila embryos., 0, 1994
Kiehart, DP; Montague, RA; Rickoll, WL; Foard, D; Thomas, GH, Methods in cell biology. 44 pp. 507-532

Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function., 1, 1993
Young, PE; Richman, AM; Ketchum, AS; Kiehart, DP, Genes & development. 7 vol. (1); pp. 29-41

Morphogenesis in Drosophila requires nonmuscle myosin heavy chain function, 0, 1993
Young, PE; Richman, AM; Ketchum, AS; Kiehart, DP, Genes and Development. 7 vol. (1); pp. X2-X3

The regulatory light chain of nonmuscle myosin is encoded by spaghetti-squash, a gene required for cytokinesis in Drosophila., 6, 1991
Karess, RE; Chang, XJ; Edwards, KA; Kulkarni, S; Aguilera, I; Kiehart, DP, Cell. 65 vol. (7); pp. 1177-1189

Dynamic changes in the distribution of cytoplasmic myosin during Drosophila embryogenesis., 1, 1991
Young, PE; Pesacreta, TC; Kiehart, DP, Development (Cambridge). 111 vol. (1); pp. 1-14

Contractile and cytoslteletal proteins in drosophilil embryogenesis, 0, 1991
Kiehart, DP, Current Topis in Membranes. 38 vol. (C); pp. 79-97

Complete sequence of the Drosophila nonmuscle myosin heavy-chain transcript: conserved sequences in the myosin tail and differential splicing in the 5' untranslated sequence., 8, 1990
Ketchum, AS; Stewart, CT; Stewart, M; Kiehart, DP, Proceedings of the National Academy of Sciences of USA. 87 vol. (16); pp. 6316-6320

Molecular genetic dissection of myosin heavy chain function., 2, 1990
Kiehart, DP, Cell. 60 vol. (3); pp. 347-350

Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites., 12, 1990
Rimm, DL; Kaiser, DA; Bhandari, D; Maupin, P; Kiehart, DP; Pollard, TD, The Journal of Cell Biology. 111 vol. (6 Pt 1); pp. 2405-2416

A beta-spectrin isoform from Drosophila (beta H) is similar in size to vertebrate dystrophin., 11, 1990
Dubreuil, RR; Byers, TJ; Stewart, CT; Kiehart, DP, The Journal of Cell Biology. 111 vol. (5 Pt 1); pp. 1849-1858

The actin membrane skeleton in Drosophila development., 10, 1990
Kiehart, DP, Seminars in cell biology. 1 vol. (5); pp. 325-339

A β-spectrin isoform from Drosophila (β<sub>H</sub>) is similar in size to vertebrate dystrophin, 0, 1990
Dubreuil, RR; Byers, TJ; Stewart, CT; Kiehart, DP, The Journal of Cell Biology. 111 vol. (5); pp. 1849-1858

Contractile proteins in Drosophila development., 0, 1990
Kiehart, DP; Ketchum, A; Young, P; Lutz, D; Alfenito, MR; Chang, XJ; Awobuluyi, M; Pesacreta, TC; Inoué, S; Stewart, CT, Annals of the New York Academy of Sciences. 582 pp. 233-251

Identification of functional regions on the tail of Acanthamoeba myosin-II using recombinant fusion proteins. I. High resolution epitope mapping and characterization of monoclonal antibody binding sites, 0, 1990
Rimm, DL; Kaiser, DA; Bhandari, D; Maupin, P; Kiehart, DP; Pollard, TD, The Journal of Cell Biology. 111 vol. (6 PART 1); pp. 2405-2416

Drosophila spectrin: the membrane skeleton during embryogenesis., 5, 1989
Pesacreta, TC; Byers, TJ; Dubreuil, R; Kiehart, DP; Branton, D, The Journal of Cell Biology. 108 vol. (5); pp. 1697-1709

Identification of the gene for fly non-muscle myosin heavy chain: Drosophila myosin heavy chains are encoded by a gene family., 3, 1989
Kiehart, DP; Lutz, MS; Chan, D; Ketchum, AS; Laymon, RA; Nguyen, B; Goldstein, LS, EMBO Journal. 8 vol. (3); pp. 913-922

Drosophila spectrin. II. Conserved features of the alpha-subunit are revealed by analysis of cDNA clones and fusion proteins., 11, 1987
Byers, TJ; Dubreuil, R; Branton, D; Kiehart, DP; Goldstein, LS, The Journal of Cell Biology. 105 vol. (5); pp. 2103-2110

Drosophilia spectrin. I. Characterization of the purified protein., 11, 1987
Dubreuil, R; Byers, TJ; Branton, D; Goldstein, LS; Kiehart, DP, The Journal of Cell Biology. 105 vol. (5); pp. 2095-2102

Cytoplasmic myosin from Drosophila melanogaster., 10, 1986
Kiehart, DP; Feghali, R, The Journal of Cell Biology. 103 vol. (4); pp. 1517-1525

Antibody inhibitors of nonmuscle myosin function and assembly., 0, 1986
Kiehart, DP; Kaiser, DA; Pollard, TD, Methods in Enzymology. 134 vol. (C); pp. 423-453

Characterization of monoclonal antibodies to Acanthamoeba myosin-I that cross-react with both myosin-II and low molecular mass nuclear proteins, 0, 1986
Hagen, SJ; Kiehart, DP; Kaiser, DA; Pollard, TD, Journal of Cell Biology. 103 vol. (6 I); pp. 2121-2128

[41] Antibody inhibitors of nonmuscle myosin function and assembly, 0, 1986
Kiehart, DP; Kaiser, DA; Pollard, TD, Methods in Enzymology. 134 vol. (C); pp. 423-453

Myosin from human erythrocytes., 1, 1985
Wong, AJ; Kiehart, DP; Pollard, TD, The Journal of biological chemistry. 260 vol. (1); pp. 46-49

Monoclonal antibodies demonstrate limited structural homology between myosin isozymes from Acanthamoeba., 9, 1984
Kiehart, DP; Kaiser, DA; Pollard, TD, The Journal of Cell Biology. 99 vol. (3); pp. 1002-1014

Direct localization of monoclonal antibody-binding sites on Acanthamoeba myosin-II and inhibition of filament formation by antibodies that bind to specific sites on the myosin-II tail., 9, 1984
Kiehart, DP; Kaiser, DA; Pollard, TD, The Journal of Cell Biology. 99 vol. (3); pp. 1015-1023

Inhibition of acanthamoeba actomyosin-II ATPase activity and mechanochemical function by specific monoclonal antibodies., 9, 1984
Kiehart, DP; Pollard, TD, The Journal of Cell Biology. 99 vol. (3); pp. 1024-1033

Stimulation of Acanthamoeba actomyosin ATPase activity by myosin-II polymerization., April 26, 1984
Kiehart, DP; Pollard, TD, Nature. (April 26, 1984) 308 vol. (5962); pp. 864-866

Temporal sequence and spatial distribution of early events of fertilization in single sea urchin eggs., 11, 1984
Eisen, A; Kiehart, DP; Wieland, SJ; Reynolds, GT, The Journal of Cell Biology. 99 vol. (5); pp. 1647-1654

Evidence that myosin does not contribute to force production in chromosome movement., 7, 1982
Kiehart, DP; Mabuchi, I; Inoué, S, The Journal of Cell Biology. 94 vol. (1); pp. 165-178

Actin and myosin function in acanthamoeba., 11, 1982
Pollard, TD; Aebi, U; Cooper, JA; Fowler, WE; Kiehart, DP; Smith, PR; Tseng, PC, Philosophical Transactions B. 299 vol. (1095); pp. 237-245

Microinjection of echinoderm eggs: apparatus and procedures., 0, 1982
Kiehart, DP, Methods in cell biology. 25 Pt B pp. 13-31

Studies on the in vivo sensitivity of spindle microtubules to calcium ions and evidence for a vesicular calcium-sequestering system., 3, 1981
Kiehart, DP, The Journal of Cell Biology. 88 vol. (3); pp. 604-617

Polymerization of actin. IV. Role of Ca++ and H+ in the assembly of actin and in membrane fusion in the acrosomal reaction of echinoderm sperm., 5, 1978
Tilney, LG; Kiehart, DP; Sardet, C; Tilney, M, The Journal of Cell Biology. 77 vol. (2); pp. 536-550

Growth and lability of Chaetopterus oocyte mitotic spindles isolated in the presence of porcine brain tubulin., 7, 1974
Inoué, S; Borisy, GG; Kiehart, DP, The Journal of Cell Biology. 62 vol. (1); pp. 175-184

Cell structure and dynamics - Editorial overview, 0, 0
DP Kiehart and K Bloom, Current Opinion in Cell Biology. {19} vol. ({1}); pp. 1-4

Book Chapters:

Ultraviolet Laser Microbeam for Dissection of Drosophila Embryos in Cell Biology: A Laboratory Handbook, 2006
Kiehart, DP; Tokutake, Y; Chang, MS; Hutson, MS; Wiemann, J; Peralta, XG; Toyama, Y; Wells, AR; Rodriguez, A; Edwards, GS

Quantitative microinjection of Drosophila embryos. in Drosophila Protocols, 2000
DP Kiehart, J.M. Crawford and R.A. Montague

Purification of Myosin and Actin from Muscle and Nonmuscle Cells in Cells, A Laboratory Manual, 1998
DP Kiehart

Contractile and Cytoskeletal Proteins in Drosophila Embryogenesis in Current Topics in Membranes, 1991
DP Kiehart

Papers Submitted

Cell Junctions and the Tension Sensitive, Supracellular Purse Strings in Drosophila Dorsal Closure in J. Cell Biol. June 20, 2012
Tulu, US; Beckerle, MC; Kiehart, DP

Ion channels function in the mechanoregulation of cell sheet forces during Drosophila morphogenesis in Developmental Cell May, 2012
Hunter, G; Crawford, J; Genkins, J; Kiehart, DP

Published Abstracts

Investigating the role of crinkled (ck) Myosin VIIA in the morphogenesis of actin-rich cellular projections in Drosophila melanogaster in Drosophila Research Conferences March 8, 2007
V Singh, JD Franke, M Chee & DP Kiehart

The contribution of the contractile actomyosin purse-string to dorsal closure during Drosophila morphogenesis investigated by UV laser microsurgery. in Biophysical Journal January, 2007
A Rodriguez-Diaz, DL Abravanel, GS Edwards & DP Kiehart

Quantitative analysis of morphogenesis during the transition of Drosophila embryos to dorsal closure in Biophysical Journal January, 2007
AR Wells & DP Kiehart

Imaging Drosophila development in FASEB Journal March, 2005
D Kiehart

Contribution of the contractile actomyosin purse-string to dorsal closure during Drosophila embryogenesis investigated by UV laser microsurgery in Molecular Biology of the Cell November, 2004
A Rodriguez, GS Edwards, DP Kiehart

Myosin VIIa is important structurally and physiologically for drosophila auditory mechanotransduction in Molecular Biology of the Cell November, 2004
SV Todi, JD Franke, DP Kiehart, DF Eberl

Force regulation during dorsal closure in Drosophila in Molecular Biology of the Cell 2004
XG Peralta, Y Toyama, A Wells, Y Tokutake, MS Hutson, S Venakides, DP Kiehart, GS Edwards

New probes for the role of non-muscle myosin II during development in Molecular Biology of the Cell November, 2002
JD Franke and DP Kiehart

Measuring the forces that drive morphogenesis: Laser-microsurgery and quantitative modeling applied to dorsal closure in Drosophila in Molecular Biology of the Cell November, 2002
S Hutson, Y Tokutake, M Chang, JW Bloor, S Venakides, DP Kiehart, GS Edwards

A molecular and genetic analysis of the forces required for morphogenesis in Journal of General Physiology July, 2001
DP Kiehart, JW Bloor, JM Wiemann, NJ Gerald, VS Williams, JD Franke, RA Montague

RhoA function in the Drosophila epidermis in Molecular Biology of the Cell November, 2001
JW Bloor and DP Kiehart

{RhoA is required for actomyosin purse string contractility in Drosophila wound healing} in Molecular Biology of the Cell November, 2001
JM Wiemann, JD Franke, DP Kiehart

Drosophila Stretchin-Mlck is a novel member of the Titin/Myosin light chain kinase family in Molecular Biology of the Cell December, 2000
MB Champagne, KA Edwards, HP Erickson, DP Kiehart

Dual labeling of the fibronectin matrix and actin cytoskeleton with green fluorescent protein variants in Molecular Biology of the Cell December, 2000
T Ohashi, DP Kiehart, HP Erickson

The role of nonsarcomeric myosin in Drosophila myogenesis. in Developmental Biology June 1, 1998
JW Bloor and DP Kiehart

Nonsarcomeric myosin II, PS2 integrin and myogenesis in Molecular Biology of the Cell November, 1998
JW Bloor and DP Kiehart

GFP-moesin fusion proteins function as real-time markers of actin cable reorganization in cultured cells. in Molecular Biology of the Cell November, 1998
DA Alcorta, RA Montague, KA Edwards, DP Kiehart

Evidence that crinkled, mutations in which cause numerous defects in Drosophila morphogenesis, encodes a myosin VII. in Molecular Biology of the Cell November, 1998
DP Kiehart, RA Montague, J Roote, M Ashburner

Forces for dorsal closure in Drosophila are both parallel and perpendicular to the morphogenic movements that cause the lateral epidermis to spread over the amnioserosa. in Molecular Biology of the Cell November, 1998
DP Kiehart, C Galbraith, RA Montague

Cellularization in Drosophila melanogaster is regulated by the Rho subfamily signaling cascades in Molecular Biology of the Cell November, 1998
JM Crawford, N Harden, T Leung, L Lim, DP Kiehart

Dynamics and elasticity of the fibronectin matrix in living cell culture visualized by fibronectin-FGP in Molecular Biology of the Cell November, 1998
T Ohashi, DP Kiehart, HP Erickson

Osmotically induced volume changes of freshly isolated rat hippocampal neurons. in FASEB Journal March 8, 1996
GG Somjen, WJ Wadman, A Juta, A Borgdorf, PG Aitken, DP Kiehart

Phosphorylation of Drosophila nonmuscle myosin II heavy chain by Protein Kinase C, Casein Kinase II and a putative myosin heavy chain kinase. in Molecular Biology of the Cell December, 1996
Z Su and DP Kiehart

Identification of in vivo interactions with Drosophila nonmuscle myosin. in Molecular Biology of the Cell December, 1996
SR Halsell and DP Kiehart

BetaHeavy-Spectrin is polarized in the membrane skeleton and essential for Drosophila development in Molecular Biology of the Cell November, 1995
GH Thomas and DP Kiehart

A screen for Drosophila cell-shape determinents that alters S. pombe morphology in Molecular Biology of the Cell November, 1995
M Foss, BY Lee, DP Kiehart

Characterization of conventional nonmuscle Myosin-II in Caenorhabditis elegans in Molecular Biology of the Cell November, 1995
EC Newbern, J Baker, DP Kiehart

Myofibrillogenesis in primary cultures of Drosophila myoblasts in Molecular Biology of the Cell November, 1995
J Ayoob, DP Kiehart, JM Sanger, JW Sanger

A GFP-Moesin fusion provides a novel tool to study changes in cell morphology in Molecular Biology of the Cell November, 1995
K Edwards, M Demsky, DP Kiehart

Cellularization in Drosophila-melanogaster is disrupted by the inhibition of RHO Activity in Molecular Biology of the Cell November, 1995
JM Crawford and DP Kiehart

Beta(Heavy)-Spectrin is polarized in the membrane skeleton and essential for Drosophila development in Molecular Biology of the Cell November, 1995
GH Thomas and DP Kiehart

Identification of cDNAs encoding Drosophila cytoskeletal elements by functional expression cloning in Schizosaccharomyces pombe in Molecular Biology of the Cell September, 1992
KA Edwards, S Shepard, B Edgar, RL Erikson, DP Kiehart

Mutations in the karst (kst) gene of Drosophila melanogaster alter the BetaHeavy-Spectrin protein and disrupt development in Molecular Biology of the Cell September, 1992
GH Thomas and DP Kiehart

The genomic DNA structure of the Drosophila cytoplasmic myosin heavy chain gene in Molecular Biology of the Cell 1992
SG Mansfield, PE Young, DP Kiehart

Nonmuscle myosin is required for cell shape change during cell sheet morphogenesis and cytokinesis in Abstracts of Papers of the American Chemical Society August 25, 1991
DP Kiehart

Nonmuscle myosin in development in Journal of Muscle Research and Cell Motility December, 1990
DP Kiehart

Profilin from Drosophila in Biophysical Journal February, 1986
AS Ketchum and DP Kiehart

Drosophila cytoplasmic myosin in Biophysical Journal February, 1986
DP Kiehart and R Feghali

Drosophila spectrin in Journal of Cell Biology November, 1986
TJ Byers, RR Dubreuil, DP Kiehart, D Branton, LSB Goldstein

Identification and partial characterization of a portion of the coding sequence for cytoplasmic myosin in Drosophila in Journal of Cell Biology November, 1986
DP Kiehart, MS Saft, RA Laymon, LSB Goldstein, J Obrien

Monoclonal antibodies as probes to the structure and function of myosin-I in Acanthamoeba in Journal of Cell Biology November, 1984
SC Hagen, DP Kiehart, DA Kaiser, TD Pollard

Monoclonal antibodies to human platelet mysoin demonstrate conserved epitopes among heterologous mysoins in Journal of Cell Biology 1984
AJ Wong, DA Kaiser, TD Pollard, DP Kiehart

Calcium transients during fertilization in single sea urchin eggs in Biological Bulletin September, 1983
A Eisen, GT Reynolds, S Wieland, DP Kiehart

Monoclonal antibodies reveal that the actin-activated ATPase of Acanthamoeba myosin-II is activated 5-10 fold by assembly of myosin-II into filaments in Journal of Cell Biology 1983
DP Kiehart and TD Pollard

Monoclonal antibodies inhibit the actin-activated Mg++ ATPase of Acanthamoeba myosin II in Biophysical Journal February, 1982
DP Kiehart, DA Kaiser, TD Pollard

Monoclonal antibodies probe myosin function in vitro and in vivo in Journal of Cell Biology November, 1982
DP Kiehart, DA Kaiser, WE Fowler, TD Pollard

Covalent modification of anti-myosin with heavy atoms for use in STEM in Journal of Cell Biology November, 1981
IM Herman, C Stoeckert, DP Kiehart, W Wiggins, M Beer, TD Pollard

Monoclonal antibodies to Acanthamoeba myosins in Journal of Cell Biology November, 1981
DP Kiehart, DA Kaiser, TD Pollard

A monoclonal antibody to myosin in Federation Proceedings 1980
DP Kiehart and TD Pollard

Monoclonal antibodies to muscle myosin in European Journal of Cell Biology 1980
DP Kiehart and TD Pollard

In vivo analysis of mitotic spindle dynamics in Journal of Supramolecular Structure 1978
S Inoue and DP Kiehart

Calcium transients during early development in echinoderms and teleosts in Biological Bulletin September, 1977
DP Kiehart, GT Reynolds, A Eisen

Cyclic contraction and relaxation of sarcomeres in isolated myofibrils in Biophysical Journal Spring, 1977
MS Mooseker, M Pratt, DP Kiehart, RE Stephens

Evidence that force production in chromosome movement does not involve myosin in Journal of Cell Biology Fall, 1977
DP Kiehart, S Inoue, I Mabuchi

Cyclic contraction and relaxation of glycerinated myofibrils isolated from skeletal muscle in Biological Bulletin Fall, 1976
MM Pratt, MS Mooseker, DP Kiehart, RE Stephens

Local depolymerization of spindle microtubules by microinjection of calcium ions in Journal of Cell Biology 1976
DP Kiehart and S Inoue

Subunit interactions of fish hemoglobins in Biological Bulletin September, 1975
C Markowitz, D Beswick, D Kiehart, D Powers

Microtubule depolymerization in local regions of mitotic spindle by Ca++ microinjection in Biological Bulletin September, 1975
D Kiehart and S Inoue

Awards

  • 2011 Elected Fellow of the American Association for the Advancement of Science, American Association for the Advancement of Science Council

  • 2011 AAAS Fellows, American Association for the Advancement of Science, The