Zeiss LSM 410 confocal microscope

The 410 confocal has permanently retired and this information is left here solely for reference. Some parts of the system were used as the base of the 2 photon

Duke University Light Microscopy Core Facility Zeiss LSM 410 confocal microscope Capabilities:
  • Imaging UV and VIS fluorophores (eg Dapi, GFP, Alexa 488/543/633, Cy2/3/5)
  • Ideal for fixed specimens (oil objectives)
  • Z-sectioning

Location: Room 4228 French Family Science Center (613-8097)

Cost: $20 per hour

User manual: pdf | html

Detailed specifications

  • Zeiss Axiovert 100 inverted microscope stand
  • Zeiss mercury arc lamp housing & power supply
Conventional fluorescence filters in cube slider:
  • UV/multi
  • Green
  • Red
Lasers:
  • Coherent Enterprise (351, 364 nm)
  • Omnichrome KrAr (488, 568 nm)
  • HeNe (633 nm)

Objectives:

  • 10x/0.50 (Zeiss Fluar)
  • 20x/0.50 Ph2 (Zeiss Plan-NeoFluar)
  • 25x/0.80 multi-immersion (Zeiss Plan-NeoFluar, in storage drawer)
  • 40x/1.30 oil DIC (Zeiss Plan Neo-Fluar)
  • 63x/1.40 oil (Plan Apochromat)
  • 100x/1.40 oil (Zeiss Plan Apochromat, in storage drawer)
  • 100x/1.30 oil (Zeiss Plan Neo-Fluar, in storage drawer)

Dichroics:

  • DBS1 UV/488/568 triple, NT 80/20, UV/488/568/633 quad
  • DBS2 LP560, LP630, open
  • DBS3 open, LP460, LP560

PMTs:

  • R/FL1 - cooled
  • R/FL2 - cooled
  • R/FL3 - standard

Emission Filters: Dichroic/filter combinations: gif pdf

PMT1 emission filters

  • LP 400 (DAPI)
  • BP 470-500 (INDO)
  • LP 505 (GFP, FITC, Alexa 488)
  • LP 510-560 (GFP, FITC, Alexa 488)
  • LP 590 (CY3/Rhod/TxRd)
  • BP 575-635 (CY3/Rhod/TxRd)
  • BP 615-645 (TxRed)
  • BP 670-810 (CY5)

PMT2 emission filters

  • BP 400-440 (DAPI)
  • BP 390-420 (INDO)
  • BP 500-530 (GFP, FITC, Alexa 488)
  • BP 500-540 (GFP, FITC, Alexa 488)
  • BP 510-560 (GFP, FITC, Alexa 488)
  • BP 580-620 (Rhod/CY3/TxRd)
  • BP 575-650 (CY3/TxRd)

PMT3 emission filters

  • LP 400 (DAPI)
  • BP 400-440 (DAPI)
  • BP 460-500 (INDO)
  • BP 500-530 (GFP, FITC, Alexa 488)
  • BP 500-540 (GFP, FITC, Alexa 488)
  • BP 510-560 (GFP, FITC, Alexa 488)
  • BP 575-650 (CY3/TxRd)

Computer:

  • Zeiss-built Pentium Pro 6 GB hard drive; 256 MB RAM
  • ZIP & CD drives disconnected
  • MS Windows95
  • Zeiss LSM software ver3.99