The photographs for this project were taken by students in the Entomology course (Biology 222L). For macrophotography we use a variety of hand-held cameras, primarily Nikon Coolpix models.
For extreme close-up photography we use two microscope setups: a ProgRes C3 (Jenoptik) digital camera mounted on a Wild M5A stereo-microscope, and an Olympus DP71 digital camera mounted on an Olympus sZX16 stereo-microscope.
We use two kinds of focus enhancement software programs: iSolution Lite (iMTechnology) and MicroSuite Five (Olympus). These programs combine the in-focus portions of a z-series of photographs, which allows one to achieve a greater depth-of-field than can be obtained with a single picture.
Most pictures were processed with Adobe Photoshop to improve brightness, contrast and color saturation, and to remove blemishes.
Scanning electron micrographs were taken with a Philips XL30 ESEM at Duke’s Shared Material & Instrumentation Facility (SMIF), of specimens sputter-coated with gold-palladium.